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report to the IT Board on overall initiative progress with metric views across af liates and perhaps neutral highlighting of best and worst performers can galvanize attention. Given that this is still a learning phase, there should be no explicit pressure for action to be taken as a result of ndings, but the potential power of the approach will have been demonstrated. Align Motivation to Scorecards (Step 5): While benchmarking implicitly places some peer pressure on IT organizations to improve, formal scorecard targets should still be agreed for af liates. In turn, performance towards these targets needs to be linked to personal target setting and incentive schemes to ensure alignment between scorecard and personal objectives. Eighty- ve per cent of all successful scorecard projects do this.6 At the very least, personal objectives should be balanced similarly to the scorecard de nition. The exact approach depends both on the management processes within a rm and the business culture, but it remains important that this alignment takes place and that dates for commencing are agreed by the IT Board. Wherever possible, target setting and reviews should be conjoint with established budget setting processes both to avoid repeat work and to contribute to the institutionalization of scorecards. Note that in the absence of historic data on some metrics, IT management will need to be exible on how binding initial targets are, as in many cases only a best guess can be made on sensible targets.

2 2 2 Rss = diag{ s1 (k), s2 (k), . . . , sn (k)}, 2 where si (k) = E{s2 (k)} means variance of the source signal si . i

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toxic effect as long as it is sequestered in the cytoplasm By contrast, co-expression of both lysin and holin (or even holin by itself) can lead to marked toxicity Not only are lysins and holins expressed together during phage infection, but they are often encoded adjacent to one another in phage genomes As a result, lysin-containing fragments in shotgun libraries commonly encode holins as well, creating the potential for selective toxicity of exactly the clones one hopes to identify When a lysinencoding clone is identi ed in an enzyme-based screen, it is generally one in which either (a) the holin and lysin happen not to be encoded adjacently in the particular genome, (b) the holin (fortuitously) is not suf ciently expressed, or (c) only a limited amount of genomic DNA surrounds the lysin, excluding the complete holin.

Due to the small size of phage genomes and the resultant high proportion of lysin-encoding clones, the issue of holin toxicity has not proven to be a tremendous obstacle in past screens Nevertheless, several studies have looked to avoid the situation altogether by instead selecting for holin-encoding clones By targeting the holin genes, it is possible to identify adjacent lysins without actually observing lysin activity In this regard, Delisle et al (2006) utilized a plasmid release protocol to identify a phage lytic enzyme for the putative dental pathogen Actinomyces naeslundii For this study, mixed E coli transformants were grown in a single-liquid culture Following induced expression, holin-encoding cells would undergo lysis, releasing their plasmid into the culture media The plasmids were then puri ed and used to retransform a new set of competent E coli.

Bahai. A.S. [470]............................ 538 Bahl. L.R. [l621 . 125. 178.418.454.481.577.600. 653. 655 Baier. A . [553] .............................. 658 Baier. P.W. [226] ....................... .279. 504 Baier. P.W. [208] ..... 248.277.280. 281.510. 525 .280. 510 Baier. P.W. [228] ....................... 1. 498 Balachandran. K . [3] ...................... Bar-Ness. Y. [405] ........................... 516 590 Barbarossa. S. [528] ......................... 128 Barbulescu. A.S [l651 ....................... Barton. S.K. [M51 ........................... 537 537 Barton. S.K. [444] ........................... Bauch. G. [ 3 11J ...........455.457459.493, 658 600. 627 Bauch. G. [532] ........................ Bauch. G . [319] . . . . . . . . . ..485.590.594.636. 648 Bauch. G . [523] . . . . . . . . . . . . . . . 590.594.636. 648 32 Beaulieu. N.C. [ 11 l] .......................... 32 Beaulieu. N.C. [ 1101 .......................... 31 Belfiore. C.A. [l021........................... Bello. P.A. [426] ............................ 536 Bergmans. J.W.M. [114J ...................... 32 Berr0u.C. [411] ............................. 517 Berrou. C. [l551 ............... 124. 173.417. 577 Berrou. C. [l531 ...... 124. 165. 166.453455. 476 Berrou. C. [l521 . 124.249.340.417.419.453.522. S77 Berruto. E . [300] ............................ 399

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