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11. Far. If the selective locus is near, it passes into the F cell very early during conjugation. Consequently, there is a great reduction in the recovery of loci distal to the selective marker because both the Hfr and F members of a conjugation event can be killed by the selective agent (e.g., an antibiotic such as streptomycin). 13. See gures 7.8, 7.9, 7.15, 7.17, 7.18, and 7.26. 15. 9 min 1 min 8 min 7 min Origin az ton lac galB 17. For example, use an Hfr that is wild-type but str with the F factor integrated at minute 20. Use an F strain that is pyrD , purB , man , uvrC , his , and strr. Interrupt mating at one-minute intervals; plate cells on complete medium with streptomycin to kill Hfr cells and grow recombinant and nonrecombinant F cells. The next day, after colonies have grown up, replica-plate onto selective media. The following data would be generated:

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ASP . NET web development; Updated: 21 May 2019.

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Known Quantities: Generator ratings and machine parameters Find: Eb , ka , T Schematics, Diagrams, Circuits, and Given Data:

Generator ratings: 1,000 kW; 2,000 V; 3,600 rev/min Circuit parameters: Ra = 01 ; ux per pole = = 05 Wb

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c. lys val his lys val his Both lys and his must be present to allow growth. d. lys val his lys val his We see no lys val his cells. e. lys and val are close together; they are cotransformed 75% of the time. Order could be lys val his or val lys his. f. val lys his. If the order is val lys his, val lys his should be rare, since this genotype results from a double exchange; and indeed, this class is the least frequent. 27. Mix the phages together with bacteria with increasing quantities of the two phages. Knowing the numbers of each in a particular case, it is possible to predict the proportion of cells doubly infected (product of probabilities).The recovery of recombinants should increase with that probability. In other words, recombination should occur only in doubly infected cells. 29. a 0.6 b 0.2 d 1.2 c

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comb-like structure in which cantilevered beams are interdigitated with a row of electrically charged fixed plates that are deposited on a silicon wafer The beams are also joined to form a single suspended frame, which deflects on the chip in response to acceleration An increase or a decrease in the speed makes the beams vibrate between the fixed plates, generating changes in capacitance between the separate structures These changes are sensed electrically and processed by using control electronics, which calculate how much voltage is needed to keep the beams from moving The voltage is proportional to the acceleration or deceleration the sensor is experiencing and is applied to the cantilevered beams as a balance [9] The application of the accelerometer is not only limited to the air-bag system It can be also used as antilock braking systems, active suspensions or disk drives for portable computers Other microsystems for the purpose of safety in an automobile include microgyroscopes for navigation and pressure and displacement sensors for object avoidance In an automobile engine, manifold control with pressure sensors, airflow control, exhaust gas analysis and control, crankshaft positioning, fuel pump pressure and fuel injection control, transmission

Analysis:

Small recombination frequencies should be approximately additive. Note that recombination distances are twice the value of wildtype plaques since the double mutant recombinants were not counted. Thus, the data table should be:

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19 The order is a c b, and c is close to a Genes c and a are cotransformed 76% of the time, suggesting that these two genes are very close and b is far away (a-b cotransduction is 10%, or 01) Two orders are possible: a-c b and c-a b If the rst order is correct, a b c results from a double crossover; this class should be the least frequent If the second order is correct, a single exchange between a and c would yield a b c , but this frequency should be similar to a b c , and it is not 21 thr leu pro his We see that cells that are thr are the most frequent The chance of interruption in the conjugation increases with the length of time for the mating Therefore, genes farther from the origin of transfer appear less frequently.

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