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39. Hypervariable DNA is DNA showing a great deal of interindividual variation. A RFLP (restriction fragment length polymorphism) is a polymorphism (variation) that shows up after Southern blotting and probing of restriction digests. A VNTR (variable number of tandem repeats) locus is one that is hypervariable due to unequal crossing over among the tandem repeats. VNTR loci are a hypervariable subset of RFLPs. Sequence-tagged sites are unique sites in the genome that can be ampli ed with polymerase chain reaction. Microsatellite DNA, repeats of very short segments such as CA, forms VNTR loci that are usually examined by polymerase chain reaction (PCR) if primer sequences are known. Critical Thinking Question:

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Fig 831: The definition of aspect ratio [4]

= arccos(pf ) = 0795

Q = 1429 10 sin(0795) = 102 kVAR 2 To compute the reactance needed for the power factor correction, we observe that we need to contribute a negative reactive power equal to 102 kVAR This requires a negative reactance, and therefore a capacitor with QC = 102 kVAR The reactance of such a capacitor is given by the expression: XC = and, since C= we have C= 1 1 = 1,175 F = XC 377 2258 1 XC |VL |2 (480)2 = = 2258 QC 102 105

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If this arrangement is correct, digestion with BglII, EcoRI, and PstI should yield 0.3 kb (BglII EcoRI), 0.7 kb (EcoRI PstI) 2.0 kb (Pst I BglII), and this is, in fact, observed. 29. The second possibility predicts that a 1.5-kb fragment should be seen in the double digestion. This fragment would result from digestion of the 3.5-kb EcoRI fragment with BglII. Since we don t see a 1.5-kb fragment in the double digest, the second possibility does not agree with the observed results. 31. Two of the three number 21 chromosomes present in the child came from the father, not the mother. Since the probe produces different bands in the mother and the father, all of these bands must also be present in the child. The intensity of a band is proportional to the amount of DNA present. The bands that are of paternal origin are more intense than the maternal bands. The father contributed two number 21 chromosomes. 33. For the steps in the dideoxy sequencing method, see gures 13.32 and 13.33. Use of uorescent dyes has allowed for the automation of the process and the elimination of radioactive tags. 35. The DNA can be inserted into the M13 general sequencing vector. 37. What will appear in the gel are fragments of the newly synthesized strand. Since DNA synthesis proceeds 5 3 , the 5 base will be T in the new strand. Proceed up the gel by indicating the base complementary to the sequence given.

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A second load is added to the circuit of Figure 734, as shown in Figure 735 Determine the required value of capacitance for perfect pf correction after the second load is added Draw the phasor diagram showing the relationship between the two load currents and the capacitor current

1. Sticky ends would exist if the plasmid DNA had a 3 overhang of one nucleotide residue, while the foreign DNA had a 3 overhang of its complement. The exact number of bases would not need to be the same because repair enzymes plus ligase could close the gap. For example, the plasmid could have a 3 tail of thymines added, whereas the foreign DNA could have a 3 tail of adenines added. This method (see gure next page) was called the polydA/poly-dT technique. 14 Gene Expression: Control in Prokaryotes and Phages 1. a. inducible (wild-type); repressed; f. inducible b-d. constitutive; e. neither, super-

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Modern machining processes are also used in the fabrication of MEMS and microsystems However, most of the processes are not stand-alone systems Developments are now taking place to produce MEMS devices using electrodischarge machining and laser machining For instance, Matsushita has developed a new electrodischarge machine with the capability of making very small,

1 Power factor correction capacitor 2 Phasor diagram pf1 = 07 lagging; P2 = 5 104 W; pf2 = 095 leading Schematics, Diagrams, Circuits, and Given Data: VS = 480 (0) V; P1 = 105 W;

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