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Figure 34-15: In the Save Exported File As dialog, choose Movie to Image Sequence as the Export type.

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Currently, some 26 million women are screened in the United States annually (approximately 55 million worldwide) In the United States there are 10 000 mammographyaccredited units Of these, 39% are community and/or public hospitals, 26% are private radiology practices, and 13% are private hospitals Though there are 10 000 mammography centres, there are only 2500 mammography speci c radiologists there is a worldwide shortage of radiologists and radiologic technologists (the term in the United Kingdom is radiographers) Huge numbers of mammograms are still read by non-specialists, contravening recommended practice, nevertheless continuing with average throughput rates between 5 and 100 per hour Whereas expert radiologists have cancer detection rates of 76 to 84%, generalists have rates that vary from between 8 to 98% (with varying numbers of false-positives).

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534 Initiating Cultures from the Human Fetal Gonad In the UK, the collection of human embryonic and fetal material is carried out according to code of practice guidelines issued by the Polkinghorne committee in 1989 [Polkinghorne, 1989] In addition, projects require current ethical approval, and material is only collected after written informed consent is obtained from women undergoing social (or voluntary) termination of pregnancy Our experience has been to collect the gonadal ridge from approximately 7 weeks postconception (wpc) to 11 wpc for identi cation and dissection under stereomicroscopy using sterilized equipment and vessels This practice can minimize the risk of subsequent infection; however, it is important to realize that material obtained per vagina is not sterile It is therefore our practice to isolate primary human germ cell cultures from all other cultures by using a dedicated incubator.

The number of cancers that are deemed to be visible in retrospect, that is, when the outcome is known, approaches 70% (American Journal of Roentgenology 1993) Staff shortages in mammography seem to stem from the perception that it is boring but risky : as we noted earlier, 12% of all malpractice lawsuits in the United States are against radiologists, with the failure to diagnose breast cancer becoming one of the leading reasons for malpractice litigation (AJR 1997 and Clark 1992) The shortage of radiologists is driving the development of specialist centres and technologies (computers) that aspire to replicate their skills Screening environments are ideally suited to computers, as they are repetitive and require objective measurements As we have noted, screening has already produced encouraging results However, there is much room for improvement For example, it is estimated that a staggering 25% of cancers are missed at screening.

The dissected gonadal ridge is placed in sterile HBSS and transferred to the culture hood for cell dissociation We describe two dissociation methods, both performing variably, probably re ecting a number of uncontrollable factors that include: the developmental stage of the material, the time between collection and processing, and the number of samples being processed The resulting suspension will consist of a mixture of single cells, small aggregates, and undigested larger clumps The clumps sink, allowing recovery of the single cells and smaller aggregates by pipette for plating Whereas the rst method probably yields a greater number of cells, the use of proteolytic enzymes lyses a proportion of the cells and potentially damages important surface proteins Conversely, the second method probably releases fewer cells but avoids protracted washing and resuspension and minimizes potential damage.

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