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from other adherent mesenchymal cells solely by antigen expression [Prockop et al., 2001, 2003]. Therefore, we suggest evaluating the absence of adherent and nonadherent cells, in particular of hematopoietic (CD34) or endothelial origin (CD31) [Spangrude et al., 1988; DeLisser et al., 1993; Negrin et al., 2000]. The protocols also recommend the analysis of CD71, the transferrin receptor, to assess the proliferative state of the isolated cells. The ability of hMSCs to undergo selective differentiation in response to environmental factors is documented by chondrogenic or osteogenic differentiation in chondrogenic or osteogenic culture medium, respectively, and the lack of differentiation in control medium. Similar per-cell amounts of glycosaminoglycan (GAG) and calcium measured in cells of higher passages (e.g., P1 , P3 , and P5 ) are also important as they enable the use of small initial bone marrow aspirates to obtain suf cient amounts of cells for seeding clinically sized scaffolds. Together, the capacity for expansion in the undifferentiated state and the maintained ability for subsequent chondrogenic or osteogenic differentiation establish the feasibility of using hMSCs for bone tissue engineering (Fig. 13.2, See Color Plate 8) [Meinel et al., 2004a]. how to write pdf file in asp.net c# How to Easily Create a PDF Document in ASP . NET Core Web API
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