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RNA This question really asks how double-stranded DNA can be formed from single-stranded RNA. First, we could synthesize a complementary DNA strand, then begin to make a second DNA strand complementary to the rst. While synthesizing the second DNA strand, we begin to degrade the RNA. The double-stranded DNA molecule now inserts into the host DNA. In order to get more viruses, a single-stranded RNA molecule must be made from the DNA. We would need, at a minimum, an enzyme to make a singlestranded DNA to form a hybrid with the RNA; an enzyme to degrade the RNA and to make the second, complementary DNA strand; an enzyme to cut the host DNA to allow the viral DNA to insert itself; an enzyme to ligate the two molecules; and an enzyme to make more viral RNA. The earlier functions are performed by one viral enzyme, reverse transcriptase. 23. Finding small pieces or fragments of DNA suggests the Okazaki pieces are only slowly, if at all, joined, a function of DNA ligase.The fact that not many long DNA molecules are seen also suggests that the DNA is being broken, implicating a nuclease as well. 25. It is unlikely that bases are added faster in developing embryos. So we must look for another mechanism. If there are more replicons, and hence more origins of DNA replication, each replicon will be shorter and be able to duplicate faster. Alternatively, and more likely, the process is regulated to slow down adult division.

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120 0 3565 T (Nm)

Now we have the two points necessary to construct the torque-speed curve for this motor, which is shown in Figure 1723 The power is related to the torque by the frequency of the shaft: Prated = T m = (3565) or, equivalently, P = 448 W 1 hp = 006 hp 746 W 120 2 = 448 W 60

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A DNA sequence with a seven-base inverted repeat is 5 -ATTACCGCGGTAAT-3 3 -TAATGGCGCCATTA-5 11. Footprinting is a technique in which DNA in contact with a protein is exposed to nucleases; only DNA protected by the protein is undigested. Promoters could be isolated by protection with RNA polymerase in the absence of ribonucleotides the polymerase will not move and then sequenced. 13. The superscripts of the sigma factors refer to their molecular weights (e.g., 70 is 70,000 daltons). Different sigma factors usually recognize different prokaryotic promoters. 15. 3 - G G T A G T A C T G T C T G G G A A C G A T T G C G -5 5 - C C AT C AT G A C A G A C C CTT G CT A A C G C - 3 Begin by writing the strand that is complementary to the RNA.This will be the transcribed strand. Remember, U in RNA pairs with A in DNA. Since transcription proceeds 5 3 , the 5 end of the RNA is opposite the 3 end of the DNA. 17. The bottom strand is transcribed and the molecule is arranged as 5 _________________3 3 _________________ 5 Begin by writing the RNA that could be transcribed from each strand. Since the DNA represents the beginning portion of the gene, the RNA must have an AUG to start protein synthesis. Unfortunately,

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Known Quantities: Motor ratings; operating conditions Find: T60 , torque delivered at 60-A series current Schematics, Diagrams, Circuits, and Given Data: Motor ratings: 10 hp; 115 V; full

Application of MEMS and microsystems in consumer products can be seen in optical switching and fibre-optic couplings, radiofrequency switches and tunable resonators (Figure 829) [4] High frequency circuits will benefit considerably from the advent of the RF-MEMS technology Electrical components such as inductors and tunable capacitors can be significantly improved compared to their integrated counterparts if they are made using MEMS and nanotechnology With the integration of such components, the performance of communication circuits will improve, while the total circuit area, power consumption and cost will be reduced In addition, the mechanical switch, as developed by several research groups, is a key component with a huge potential in various microwave circuits [7] Fig 829: An MEMS tunable bank [4] Motorola has introduced a pressure sensor to merge signal conditioning with mechanical sensing on a single chip The pressure sensor is well suited to be applied in microprocessor-based systems that have analogue to digital inputs [9]

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