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Assume you ve installed anti-spyware/anti-adware software on your computer (if this assumption is incorrect, you might want to jump ahead to the Top Anti-Spyware/Anti-Adware Picks section, where you can read about candidates for and the processes involved in installing this kind of software on your PC). After such software is installed, you should use it immediately to scan for possible spyware or adware infestation. This guarantees a clean start for your system going forward (or will help you clean up and restore your system to a more or less pristine state). This usually means digging into program menus and finding out how to use a program s scanning capabilities. Let s take a look at how you d do this with Spybot-Search & Destroy (as I wrote this chapter Version 1.3 had just been released; the relevance of this description will vary as version numbers change, but it should still give you an idea about what s involved in this effort). Here are the opening steps involved in scanning and starting a repair (I ll describe the rest verbally, because system restarts make it nearly impossible to capture screenshots during that process): 1. Launch Spybot-Search & Destroy. I did so by clicking Start All Programs SpybotSearch & Destroy (menu heading) Spybot-Search & Destroy (program name). You can also click Start Run, type %ProgramFiles%/Spybot - Search & Destroy/ SpybotSD.exe in the Open dialog box, and click OK. This produces the screen shown in Figure 7-1.

7-13

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Glare, the most frequent complaint among WS users, is the sensation produced within the visual eld by luminance that is suf ciently greater than the luminance to which the eyes are adapted to cause annoyance, discomfort, or loss in visual performance and visibility. Glare can be caused by re ections of electric light sources, windows, and lightcolored objects including furniture and clothing. The magnitude of the glare sensation is a function of the size, position, and luminance of a source, the number of sources, and the luminance to which the eyes are adapted at the moment. It may be categorized according to its origin: direct or re ected glare. Direct glare may be caused by bright sources of light in the visual eld of view (e.g., sunlight and lightbulbs). Re ected glare is caused by light re ected from the display screen. If the re ections are diffuse, they are referred to as veiling glare. Image re ections are both distracting and annoying, since the eye is induced to focus alternately between the displayed and re ected images. The re ected glare can be reduced by increasing the display contrast, by wearing dark-colored clothing, by correctly positioning the screen with respect to lights, windows, and other re ective objects, and by adjusting the screen angle. 12.2.2 Ambient Illuminance

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measured point spread function or through blind deconvolution This method is used in medicine for reconstruction of 3D images from CT and MRI scans Other methods are based on the measurement of local sharpness of the image (Nahm et al, 1998) These methods work best for objects with distinct lateral features, but these methods have dif culty resolving objects with smooth surfaces that have no lateral features Stereoscopic Imaging Images registered using a stereoscopic microscope are analyzed by identifying the same features in both images and measuring the distance between features Knowing the angles through which the two stereoscopic images are observed allows for the determination of the relative axial distances between different features on the object Like deconvolution, this method works best for objects with distinct lateral features, but does not work well for objects with smooth surfaces or with periodic structures.

Table 5.1. Range of separation of DNA and proteins in polyacrylamide gels of different polyacrylamide concentration Range of separation Acrylamide concentration % (w/v) 3.5 5 8 12 15 20

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Stereoscopic imaging can be used to image both large and small objects as long as the images are obtained from two different perspectives 1532 Confocal Microscopy Like many interferometric methods confocal microscopy really took off with the development of image processing software and affordable, powerful lasers and computers Back in 1961, Minsky patented modi cations to a biological microscope that reduced the stray light in the system in order to improve the obtained image; however, only in the mid 1980s did the development of confocal microscopes to obtain noninvasive, three-dimensional data from biological specimens occur Since this time, confocal systems have been an important measurement tool in the elds of cell biology, physiology, cytogenics and developmental biology (Pawley, 1995; Gu, 1996) and ophtalmology The introduction of two-photon microscopy (Diaspro, 2002) in the late 1990s offered reduced photodamage and increased tissue penetration for better imaging.

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